envelope plasmid pcmv vsv g encoding vsv g protein Search Results


vesicular stomatitis virus  (Addgene inc)
96
Addgene inc vesicular stomatitis virus
Vesicular Stomatitis Virus, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/envelope+plasmid+pcmv+vsv+g+encoding+vsv+g+protein/VSV%2EG+(Plasmid+%2314888)/pmc08787484-239-42-26
Average 96 stars, based on 1 article reviews
vesicular stomatitis virus - by Bioz Stars, 2026-11
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vesicular stomatitis virus g protein vsv g  (ATCC)
94
ATCC vesicular stomatitis virus g protein vsv g
Vesicular Stomatitis Virus G Protein Vsv G, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/envelope+plasmid+pcmv+vsv+g+encoding+vsv+g+protein/Vesicular+stomatitis+virus/pmc02586287-75-24-51
Average 94 stars, based on 1 article reviews
vesicular stomatitis virus g protein vsv g - by Bioz Stars, 2026-11
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vsv g envelope protein  (Addgene inc)
96
Addgene inc vsv g envelope protein
Vsv G Envelope Protein, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/envelope+plasmid+pcmv+vsv+g+encoding+vsv+g+protein/pCMV-VSV-G+(Plasmid+%238454)/pm34314702-590-18-22
Average 96 stars, based on 1 article reviews
vsv g envelope protein - by Bioz Stars, 2026-11
96/100 stars
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vsv g envelope protein plasmid pmd2g  (Addgene inc)
98
Addgene inc vsv g envelope protein plasmid pmd2g
Vsv G Envelope Protein Plasmid Pmd2g, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/envelope+plasmid+pcmv+vsv+g+encoding+vsv+g+protein/pMD2%2EG+(Plasmid+%2312259)/10__3233_slash_jad___190086-49-43-49
Average 98 stars, based on 1 article reviews
vsv g envelope protein plasmid pmd2g - by Bioz Stars, 2026-11
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g protein  (Addgene inc)
85
Addgene inc g protein
G Protein, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/envelope+plasmid+pcmv+vsv+g+encoding+vsv+g+protein/G+protein+alpha-s(alpha3beta5%2FG226A)+(Plasmid+%2355798)/pmc04776322-300-63-71
Average 85 stars, based on 1 article reviews
g protein - by Bioz Stars, 2026-11
85/100 stars
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vsv g envelope protein vector pcag vsv g  (ATCC)
99
ATCC vsv g envelope protein vector pcag vsv g
Vsv G Envelope Protein Vector Pcag Vsv G, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/envelope+plasmid+pcmv+vsv+g+encoding+vsv+g+protein/293/bio_rxiv__196170-66-19-28
Average 99 stars, based on 1 article reviews
vsv g envelope protein vector pcag vsv g - by Bioz Stars, 2026-11
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vsvg gfp  (Addgene inc)
93
Addgene inc vsvg gfp
Vsvg Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/envelope+plasmid+pcmv+vsv+g+encoding+vsv+g+protein/pEGFP-VSVG+(Plasmid+%2311912)/pmc08728401-44-0-2
Average 93 stars, based on 1 article reviews
vsvg gfp - by Bioz Stars, 2026-11
93/100 stars
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vsv g  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology vsv g
( A ) Schematic overview of the protocol for the differentiation of mature podocytes from human iPS cells; adapted from . ( B ) Human iPS cell-derived podocytes express the lineage specific markers nephrin (green) <t>and</t> <t>podocin</t> (magenta). Cells were counterstained with DAPI (blue) nuclear marker. Scale bar, 100 μm. ( C ) Schematic depicting the lentiviral vectors used to produce S-pseudotyped virus from 293T cells and infection of human iPS cell-derived podocytes in vitro . ( D-E ) Western blot confirming the successful transfection of 293T cells and production of S-pseudotyped virus indicated by the presence of Spike protein (190kDA) and its cleavage products S1 (∼110kDa) in both ( D ) the cell lysates (β-actin used as loading control) and ( E ) purified viral particle (normalized to HIV viral protein p24) with extra band for S2 (∼100kDa). Mock for figure D represents lysate from untransfected cells while in E, mock represents pseudoviral particle dissolving media (TBS); SARS-2-S represent lysate from cells transfected with spike expressing plasmid or S-pseudotyped particle; Bald virus represent lysate from cells transfected with no envelope plasmid or the resulting pseudovirus particle without viral envelope protein; <t>VSV-G</t> represent lysate from cells transfected with plasmid expressing VSV-G or pseudotyped particle with VSV-G envelope ( F ) Schematic showing propagation of patient-derived SARS-CoV-2 virus in Vero E6 cells followed by purification and infection of human iPS cell-derived podocytes in vitro .
Vsv G, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/envelope+plasmid+pcmv+vsv+g+encoding+vsv+g+protein/VSV-G+Antibody/bio_rxiv__2021__11__16__468893-129-20-21
Average 94 stars, based on 1 article reviews
vsv g - by Bioz Stars, 2026-11
94/100 stars
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ts045 vsvg gfp plasmids  (Thermo Fisher)
99
Thermo Fisher ts045 vsvg gfp plasmids
( A ) Schematic overview of the protocol for the differentiation of mature podocytes from human iPS cells; adapted from . ( B ) Human iPS cell-derived podocytes express the lineage specific markers nephrin (green) <t>and</t> <t>podocin</t> (magenta). Cells were counterstained with DAPI (blue) nuclear marker. Scale bar, 100 μm. ( C ) Schematic depicting the lentiviral vectors used to produce S-pseudotyped virus from 293T cells and infection of human iPS cell-derived podocytes in vitro . ( D-E ) Western blot confirming the successful transfection of 293T cells and production of S-pseudotyped virus indicated by the presence of Spike protein (190kDA) and its cleavage products S1 (∼110kDa) in both ( D ) the cell lysates (β-actin used as loading control) and ( E ) purified viral particle (normalized to HIV viral protein p24) with extra band for S2 (∼100kDa). Mock for figure D represents lysate from untransfected cells while in E, mock represents pseudoviral particle dissolving media (TBS); SARS-2-S represent lysate from cells transfected with spike expressing plasmid or S-pseudotyped particle; Bald virus represent lysate from cells transfected with no envelope plasmid or the resulting pseudovirus particle without viral envelope protein; <t>VSV-G</t> represent lysate from cells transfected with plasmid expressing VSV-G or pseudotyped particle with VSV-G envelope ( F ) Schematic showing propagation of patient-derived SARS-CoV-2 virus in Vero E6 cells followed by purification and infection of human iPS cell-derived podocytes in vitro .
Ts045 Vsvg Gfp Plasmids, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/envelope+plasmid+pcmv+vsv+g+encoding+vsv+g+protein/Transferrin/pmc01237059-66-6-37
Average 99 stars, based on 1 article reviews
ts045 vsvg gfp plasmids - by Bioz Stars, 2026-11
99/100 stars
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vsv g envelop protein  (Addgene inc)
96
Addgene inc vsv g envelop protein
( A ) Schematic overview of the protocol for the differentiation of mature podocytes from human iPS cells; adapted from . ( B ) Human iPS cell-derived podocytes express the lineage specific markers nephrin (green) <t>and</t> <t>podocin</t> (magenta). Cells were counterstained with DAPI (blue) nuclear marker. Scale bar, 100 μm. ( C ) Schematic depicting the lentiviral vectors used to produce S-pseudotyped virus from 293T cells and infection of human iPS cell-derived podocytes in vitro . ( D-E ) Western blot confirming the successful transfection of 293T cells and production of S-pseudotyped virus indicated by the presence of Spike protein (190kDA) and its cleavage products S1 (∼110kDa) in both ( D ) the cell lysates (β-actin used as loading control) and ( E ) purified viral particle (normalized to HIV viral protein p24) with extra band for S2 (∼100kDa). Mock for figure D represents lysate from untransfected cells while in E, mock represents pseudoviral particle dissolving media (TBS); SARS-2-S represent lysate from cells transfected with spike expressing plasmid or S-pseudotyped particle; Bald virus represent lysate from cells transfected with no envelope plasmid or the resulting pseudovirus particle without viral envelope protein; <t>VSV-G</t> represent lysate from cells transfected with plasmid expressing VSV-G or pseudotyped particle with VSV-G envelope ( F ) Schematic showing propagation of patient-derived SARS-CoV-2 virus in Vero E6 cells followed by purification and infection of human iPS cell-derived podocytes in vitro .
Vsv G Envelop Protein, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/envelope+plasmid+pcmv+vsv+g+encoding+vsv+g+protein/pCMV+delta+R8%2E2+(Plasmid+%2312263)/bio_rxiv__2020__07__24__205583-182-50-53
Average 96 stars, based on 1 article reviews
vsv g envelop protein - by Bioz Stars, 2026-11
96/100 stars
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packaging plasmids pg-p1-vsvg  (Shanghai GenePharma)
90
Shanghai GenePharma packaging plasmids pg-p1-vsvg
( A ) Schematic overview of the protocol for the differentiation of mature podocytes from human iPS cells; adapted from . ( B ) Human iPS cell-derived podocytes express the lineage specific markers nephrin (green) <t>and</t> <t>podocin</t> (magenta). Cells were counterstained with DAPI (blue) nuclear marker. Scale bar, 100 μm. ( C ) Schematic depicting the lentiviral vectors used to produce S-pseudotyped virus from 293T cells and infection of human iPS cell-derived podocytes in vitro . ( D-E ) Western blot confirming the successful transfection of 293T cells and production of S-pseudotyped virus indicated by the presence of Spike protein (190kDA) and its cleavage products S1 (∼110kDa) in both ( D ) the cell lysates (β-actin used as loading control) and ( E ) purified viral particle (normalized to HIV viral protein p24) with extra band for S2 (∼100kDa). Mock for figure D represents lysate from untransfected cells while in E, mock represents pseudoviral particle dissolving media (TBS); SARS-2-S represent lysate from cells transfected with spike expressing plasmid or S-pseudotyped particle; Bald virus represent lysate from cells transfected with no envelope plasmid or the resulting pseudovirus particle without viral envelope protein; <t>VSV-G</t> represent lysate from cells transfected with plasmid expressing VSV-G or pseudotyped particle with VSV-G envelope ( F ) Schematic showing propagation of patient-derived SARS-CoV-2 virus in Vero E6 cells followed by purification and infection of human iPS cell-derived podocytes in vitro .
Packaging Plasmids Pg P1 Vsvg, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/envelope+plasmid+pcmv+vsv+g+encoding+vsv+g+protein/packaging+plasmids/pmc11753773-70-0-15
Average 90 stars, based on 1 article reviews
packaging plasmids pg-p1-vsvg - by Bioz Stars, 2026-11
90/100 stars
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Image Search Results


( A ) Schematic overview of the protocol for the differentiation of mature podocytes from human iPS cells; adapted from . ( B ) Human iPS cell-derived podocytes express the lineage specific markers nephrin (green) and podocin (magenta). Cells were counterstained with DAPI (blue) nuclear marker. Scale bar, 100 μm. ( C ) Schematic depicting the lentiviral vectors used to produce S-pseudotyped virus from 293T cells and infection of human iPS cell-derived podocytes in vitro . ( D-E ) Western blot confirming the successful transfection of 293T cells and production of S-pseudotyped virus indicated by the presence of Spike protein (190kDA) and its cleavage products S1 (∼110kDa) in both ( D ) the cell lysates (β-actin used as loading control) and ( E ) purified viral particle (normalized to HIV viral protein p24) with extra band for S2 (∼100kDa). Mock for figure D represents lysate from untransfected cells while in E, mock represents pseudoviral particle dissolving media (TBS); SARS-2-S represent lysate from cells transfected with spike expressing plasmid or S-pseudotyped particle; Bald virus represent lysate from cells transfected with no envelope plasmid or the resulting pseudovirus particle without viral envelope protein; VSV-G represent lysate from cells transfected with plasmid expressing VSV-G or pseudotyped particle with VSV-G envelope ( F ) Schematic showing propagation of patient-derived SARS-CoV-2 virus in Vero E6 cells followed by purification and infection of human iPS cell-derived podocytes in vitro .

Journal: bioRxiv

Article Title: BSG/CD147 and ACE2 receptors facilitate SARS-CoV-2 infection of human iPS cell-derived kidney podocytes

doi: 10.1101/2021.11.16.468893

Figure Lengend Snippet: ( A ) Schematic overview of the protocol for the differentiation of mature podocytes from human iPS cells; adapted from . ( B ) Human iPS cell-derived podocytes express the lineage specific markers nephrin (green) and podocin (magenta). Cells were counterstained with DAPI (blue) nuclear marker. Scale bar, 100 μm. ( C ) Schematic depicting the lentiviral vectors used to produce S-pseudotyped virus from 293T cells and infection of human iPS cell-derived podocytes in vitro . ( D-E ) Western blot confirming the successful transfection of 293T cells and production of S-pseudotyped virus indicated by the presence of Spike protein (190kDA) and its cleavage products S1 (∼110kDa) in both ( D ) the cell lysates (β-actin used as loading control) and ( E ) purified viral particle (normalized to HIV viral protein p24) with extra band for S2 (∼100kDa). Mock for figure D represents lysate from untransfected cells while in E, mock represents pseudoviral particle dissolving media (TBS); SARS-2-S represent lysate from cells transfected with spike expressing plasmid or S-pseudotyped particle; Bald virus represent lysate from cells transfected with no envelope plasmid or the resulting pseudovirus particle without viral envelope protein; VSV-G represent lysate from cells transfected with plasmid expressing VSV-G or pseudotyped particle with VSV-G envelope ( F ) Schematic showing propagation of patient-derived SARS-CoV-2 virus in Vero E6 cells followed by purification and infection of human iPS cell-derived podocytes in vitro .

Article Snippet: The primary antibodies used were nephrin (Progen, GP-N2); podocin (Abcam, ab50339); anti-SARS-CoV2 spike (ProSci, 3525); anti-SARS-CoV-2 N protein (Sinobiological, 40143-R019); VSV-G (Santa Cruz Biotechnology, sc-365019); Human/Mouse/Rat/Hamster ACE-2 (R&D systems, AF933), Human TRA-1-85/CD147 (R&D systems, MAB3195), Cathepsin L (Santa Cruz Biotechnology, sc-32320), TMPRSS2 (Santa Cruz Biotechnology, sc-515727) and DC-SIGN/CD209 (Santa Cruz Biotechnology, sc-65740).

Techniques: Derivative Assay, Marker, Infection, In Vitro, Western Blot, Transfection, Purification, Expressing, Plasmid Preparation

( A ) qRT-PCR analysis using Lenti-X titration kit revealed time-dependent increase in the copies of viral RNA in human iPS cell-derived podocytes infected with the S-pseudovirus. ( B ) Western blot confirmed time-dependent increase of S-pseudovirus particles in human iPS cell-derived podocytes, where p24 (GAG) is a marker for the pseudoviral capsid protein. ( C ) Western blot from cell lysates of mock and infected (S-, Bald and VSV-G pseudotyped) podocytes confirming the presence of SARS-CoV-2 spike proteins in S-pseudotyped infected podocytes, HIV viral protein p24 visible in all pseudotyped infected cells but not in mock and β-actin used as loading control present in all cell lysates ( D ) qRT-PCR data measuring the levels of EGFP using GFP specific primers ( E ) Percentage of EGFP positive cells compared to total cell number ( F ) Immunofluorescent staining showing DAPI, EGFP and merged in pseudotyped infected cells and mock. Scale bar: 100 µm. ( G ) qRT-PCR results showing significantly higher number of S-pseudotyped copies in human iPS cell-derived podocytes compared to Calu-3 and Caco-2 cell lines, 72 hours post infection (h.p.i.). The statistical test was done by One-way ANOVA with Sidak’s multiple comparison test. Error bars indicate standard deviation of the mean. Only p values of 0.05 or lower were considered statistically significant (p > 0.05 [ns, not significant], p < 0.05 [*], p < 0.01 [**], p < 0.001 [***], p < 0.0001 [****]).

Journal: bioRxiv

Article Title: BSG/CD147 and ACE2 receptors facilitate SARS-CoV-2 infection of human iPS cell-derived kidney podocytes

doi: 10.1101/2021.11.16.468893

Figure Lengend Snippet: ( A ) qRT-PCR analysis using Lenti-X titration kit revealed time-dependent increase in the copies of viral RNA in human iPS cell-derived podocytes infected with the S-pseudovirus. ( B ) Western blot confirmed time-dependent increase of S-pseudovirus particles in human iPS cell-derived podocytes, where p24 (GAG) is a marker for the pseudoviral capsid protein. ( C ) Western blot from cell lysates of mock and infected (S-, Bald and VSV-G pseudotyped) podocytes confirming the presence of SARS-CoV-2 spike proteins in S-pseudotyped infected podocytes, HIV viral protein p24 visible in all pseudotyped infected cells but not in mock and β-actin used as loading control present in all cell lysates ( D ) qRT-PCR data measuring the levels of EGFP using GFP specific primers ( E ) Percentage of EGFP positive cells compared to total cell number ( F ) Immunofluorescent staining showing DAPI, EGFP and merged in pseudotyped infected cells and mock. Scale bar: 100 µm. ( G ) qRT-PCR results showing significantly higher number of S-pseudotyped copies in human iPS cell-derived podocytes compared to Calu-3 and Caco-2 cell lines, 72 hours post infection (h.p.i.). The statistical test was done by One-way ANOVA with Sidak’s multiple comparison test. Error bars indicate standard deviation of the mean. Only p values of 0.05 or lower were considered statistically significant (p > 0.05 [ns, not significant], p < 0.05 [*], p < 0.01 [**], p < 0.001 [***], p < 0.0001 [****]).

Article Snippet: The primary antibodies used were nephrin (Progen, GP-N2); podocin (Abcam, ab50339); anti-SARS-CoV2 spike (ProSci, 3525); anti-SARS-CoV-2 N protein (Sinobiological, 40143-R019); VSV-G (Santa Cruz Biotechnology, sc-365019); Human/Mouse/Rat/Hamster ACE-2 (R&D systems, AF933), Human TRA-1-85/CD147 (R&D systems, MAB3195), Cathepsin L (Santa Cruz Biotechnology, sc-32320), TMPRSS2 (Santa Cruz Biotechnology, sc-515727) and DC-SIGN/CD209 (Santa Cruz Biotechnology, sc-65740).

Techniques: Quantitative RT-PCR, Titration, Derivative Assay, Infection, Western Blot, Marker, Staining, Standard Deviation